o ctober 2026 un co rr ec te d ma nu sc ri pt equipment micro dissecting scissors stoelting 5213202p high precision dissecting micro scissors (Stoelting inc)
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o ctober 2026 un co rr ec te d ma nu sc ri pt equipment micro dissecting scissors stoelting 5213202p high precision dissecting micro scissors
O Ctober 2026 Un Co Rr Ec Te D Ma Nu Sc Ri Pt Equipment Micro Dissecting Scissors Stoelting 5213202p High Precision Dissecting Micro Scissors, supplied by Stoelting inc, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scissors%2C+straight+sharp%26sharp/Micro+Dissecting+Scissors%2C+Straight%2C+Sharp/pm42853188-510-594-612
Average 90 stars, based on 2 article reviews
O Ctober 2026 Un Co Rr Ec Te D Ma Nu Sc Ri Pt Equipment Micro Dissecting Scissors Stoelting 5213202p High Precision Dissecting Micro Scissors, supplied by Stoelting inc, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scissors%2C+straight+sharp%26sharp/Micro+Dissecting+Scissors%2C+Straight%2C+Sharp/pm42853188-510-594-612
Average 90 stars, based on 2 article reviews
o ctober 2026 un co rr ec te d ma nu sc ri pt equipment micro dissecting scissors stoelting 5213202p high precision dissecting micro scissors - by Bioz Stars,
2026-10
90/100 stars
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Cell-Signaling:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Recombinant:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Electron Microscopy:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Saline:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Glass:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Transferring:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Magnetic Resonance Imaging:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Modification:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Tissue:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Binding Assay:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Imaging:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Microscopy:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Incubator:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Automated:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Spinning Disk:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Inverted Microscopy:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Protease Inhibitor:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 Staining:Article Title: Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Article Snippet: Straight operating scissors: sharp/blunt blades , Article Title: Method for modeling oviduct function and impact on embryonic development†. Article Snippet: Materials Company Catalog number Comments Mouse models Wnt7aCre/+ mice Jackson Laboratory 036637-JAX Tg(Wnt7a- EGFP/cre)#Bhr/Mmj ax mT/mGf/f reporter mice Jackson Laboratory 007576 Gt(ROSA)26Sortm4(AC TB-tdTomato,-EGFP)Luo/J Antibodies Mouse monoclonal anti-ECadherin BD Biosciences 610182, RRID: AB_397581 Immunofluorescent (IF), 1:1000 Rabbit monoclonal antiVimentin Cell Signaling 5741S, RRID: AB_10695459 IF, 1:1000 Mouse monoclonal antiacetylated a-tubulin Millipore Sigma T6793, RRID: AB_477585 IF, 1:16000 Rabbit polyclonal antioviductal glycoprotein 1 (OVGP1) Invitrogen PA520580, RRID: AB_ 11157467 IF, 1:500 Alexa Fluor 488, goat antimouse IgG Jackson ImmunoResearch 115545146, RRID: AB_2307324 IF, 1:500 Alexa Fluor 594, goat antirabbit IgG Jackson ImmunoResearch 111-585-144, RRID: AB_2307325 IF, 1:500 Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Gibco 12634028 Leibovitz’s L-15 medium Gibco 41300-039 B27 Supplement Gibco 12587010 Insulin-transferrin-selenium (ITS) Gibco 41400045 Primocin InvivoGen NC9141851 Glutamax Gibco 35050061 A83-01 BioGems 9094360 Murine EGF Biotechne 2028-EG-200 Murine FGF-10 Biotechne 6224-FG-025/CF Murine R-Spondin1 Biotechne 7150-RS-250/CF Murine Noggin Biotechne 6997-NG-100/CF Murine Wnt3a Bio-Techne, R&D systems 1324-WN-500/CF Human EGF Bio-Techne AFL236-200 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Human FGF-10 Bio-Techne 345-FG/CF-MTO Human R-Spondin1 Bio-Techne 4645-RS-01M/CF Human Noggin Bio-Techne 6057-NG-100/CF Materials Company Catalog number Comments Human HGF Bio-Techne 294-HG-100/CF N-acetyl-L-cysteine Sigma A9165 Nicotinamide BioGems 9899208 N-2 Supplenet 100X Gibco 17502001 Y-27632 dihydrochloride Sigma Y0503 Antibiotic-Antimycotic (100X) Gibco 15240062 MgCl2 (1 M) Invitrogen AM9530G Cultrex BME R&D Systems 3433010-R1 Dimethyl sulfoxide (DMSO) Thermo Fisher Scientific D128 Fetal bovine serum (FBS) Gibco 16141079 Collagenase V Sigma C9263 DNAse-I Roche 10104159001 Liberase Roche 05401020001 Hyaluronidase Millipore Sigma H4272 TrypLE Express, no phenol red Gibco 12604021 Dulbecco’s Phosphate buffered saline (DPBS) Millipore Sigma D8537 Borosilicate glass Pasteur pipette VWR International 89399-924 KSOM + AA with D-Glucose EmbryoMax MR-106-D Light Mineral Oil Irvine Scientific 9305 Trypan Blue Solution Gibco 15250061 DNase/RNase-Free Distilled Water Invitrogen 10977015 Hoechst 33342 Thermo Fisher Scientific H3570 Agarose Low Melting Thermo Fisher Scientific BP165-25 Sodium citrate dihydrate Thermo Fisher Scientific BP327 Paraformaldehyde Thermo Fisher Scientific 416785000 Xylenes, histological grade Sigma-Aldrich 534056 Ethanol, 200 proof (100%) Decon Labs 2705HC Normal goat serum MP Biomedicals 08642921 ProLong Diamond Antifade Mountant Thermo Fisher Scientific 36961 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 O ctober 2026 UN CO RR EC TE D MA NU SC RI PT Hematoxylin solution, Harris modified Sigma-Aldrich HHS32 Calcium Sulfate, Drierite (Desiccant) Alfa Aesar 89751-P5 Materials Company Catalog number Comments Blue Ribbon Paraffin Leica Biosystems 3801360 ART Wide Bore Filtered Pipette Tips 200 μL Thermo Fisher Scientific 2069G ART Wide Bore Filtered Pipette Tips 1000 μL Thermo Fisher Scientific 2079G 12-Well Polystyrene Tissue Culture Plates Cell Treat 229112 48-well Ultra-Low Binding Culture Plate ScienCell 0343-4 Nunclon Sphera 96U-Well Round Bottom Plates Thermo Fisher Scientific 174929 35-mm petri dish Falcon 351008 For tissue collection 35-mm glass-bottom dish with 100 micro-well Cellvis D35-10-0-N For imaging ciliary beat frequency 25 cm2 (T-25) Polystyrene Tissue Culture Treated Flasks CellTreat 229331 1.5 mL polypropylene centrifugal tubes Axygen MCT150C 5 mL polypropylene centrifuge tubes Axygen MCT500CS 15 mL polypropylene centrifuge tubes CellTreat 229411 50 mL polypropylene centrifuge tubes CellTreat 229421 40 μm Cell strainer Corning 352340 500 mL Filter System, 0.22 μm PES Filter CellTreat 229707 Tissue-Tek Cryomold Sakura 4565 Tissue-Tek Mega Cassette System Sakura 4173 FisherBrand Superfrost Plus Adhesion Microscope Slides Thermo Fisher Scientific 1255015 SecureSeal Imaging Spacer Electron Microscopy Sciences 70327-9S Microscope coverglass No.1 24×55 mm Thermo Fisher Scientific 12541033 Microscope coverglass No.1 18 × 18 mm Thermo Fisher Scientific 12541012 E-3063 tubing Tygon ACF00011 academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag225/8898644 by BIR EM E / U N IFESP user on 19 |
